to enzymatic plasmid DNA amplification
Simply mix your
into the tube reaction
Switching system provides you to switch the plasmid DNA amplification from using living cells to cell-free system.
SS OriC Cassettes can be inserted by a two-step isothermal enzymatic reaction toenable cell-free amplification
Open circular DNA
・Skip primer design, Just putting your plasmid into the tube
・Applicable to pUC-, pET- and pGEM-based vectors of 4-13 kb
・No need to make glycerol stock
・Achieve up to 1 µg supercoiled DNA per 10 µL reaction
The art of SS OriC Cassettes
SS OriC Cassette was inserted into plasmid DNA using
Cell-Free Switching System, and it was amplified cell-free.
4 kb : pUC-based vector with 4K size
7 kb : pET-based vector with 1.8K genes inserted
13 kb: OriCiro's original circular DNA
- :Plasmid DNA before Switching System reaction
+: Plasmid DNA after Switching System reaction
Check your vector
SS OriC Cassettes, which have overlapping ends to the target site commonly present in pUC, pET and pGEM based plasmids.