to enzymatic plasmid DNA amplification


Simply mix your 

plasmid DNA

into the tube reaction

Switching system provides you to switch the plasmid DNA amplification from using living cells to cell-free system. 

SS OriC Cassettes can be inserted by a two-step isothermal enzymatic reaction toenable cell-free amplification


Open circular DNA


・Skip primer design, Just putting your plasmid into the tube
・Applicable to pUC-, pET- and pGEM-based vectors of 4-13 kb
・Endotoxin free
・No need to make glycerol stock
・Achieve up to 1 µg supercoiled DNA per 10 µL reaction

The art of SS OriC Cassettes

SS OriC Cassette was inserted into plasmid DNA using
Cell-Free Switching System, and it was amplified cell-free.

4 kb : pUC-based vector with 4K size
7 kb : pET-based vector with 1.8K genes inserted
13 kb: OriCiro's original circular DNA

- :Plasmid DNA before Switching System reaction
+: Plasmid DNA after Switching System reaction



Check your vector

SS OriC Cassettes, which have overlapping ends to the target site commonly present in pUC, pET and pGEM based plasmids.


OriCiro Genomics K.K
 318 South Clinical Research Bldg. 7-3-1, Hongo, Bunkyo, Tokyo 113-8485, Japan