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Cell-free synthesis and amplification of genome-scale large DNA

01 SUMMARY

Cell-free assembly and amplification aimed at replacing cell-based cloning

OriCiro offers ground breaking, cell-free technologies of large DNA assembly and amplification that can effectively replace the conventional E. coli cloning process.

02 ASSEMBLY

Hyper assembly of multiple DNA fragments

  • One-step isothermal reaction, cell-free, easy-to-handle
  • Seamless assembly via overlapping homologous ends (25 - 60 bp)
  • Simultaneous assembly of up to 50 fragments
  • Assembly of large DNA fragments up to 200 kb

03 AMPLIFICATION

In-vitro reconstitution of chromosome replication cycle

  • Whole replication cycle of the E. coli chromosome is reconstituted in vitro using purified proteins.
  • The replication cycle repeats autonomously and continuously allowing to "exponential" amplification of circular DNA.
  • Requirements for the template DNA are "circular configuration" and "oriC sequence (300 bp)."

Link to the relevant scientific publication

Cell-free hyper amplification of large DNA

  • One-step isothermal reaction, cell-free, easy-to-handle
  • Up to 100 ng/µl supercoiled DNA produced within 3 hours at 30ºC even from a single DNA molecule (for 10 kbp DNA)
  • Extremely high fidelity
  • Applicable to a wide range of DNA size from 1 kbp to 1 Mbp
  • Applicable to any sequence including GC-rich and repeat sequences

04 COMBINATION

Efficient cell-free production of circular DNA from multiple DNA fragments

  • Apply the assembled products directly to the amplification reaction.
  • Circular DNA (complete assembled products) can be amplified selectively from a single molecule level.
  • No need of purification process after assembly as unnecessary intermediates (linear DNA) are not amplified.

05 STRENGTH

Distinctive advantages of our amplification technology

OriCiro technology has a number of advantages over existing approaches making our technology very unique and competitive.
PCR E. coli cloning OriCiro Technology
DNA size +
(10 kbp)		 ++
(300 kbp)		 +++
(1 Mbp)
Operation Thermocycler Cumbersome process
Several days requiring techniques		 Very simple process
Several hours of
isothermal incubation
Biosafety Cell-free Recombinant DNA experiment Cell-free
Fidelity +
(10-4~10-6 error/bp)		 +++
(10-10 error/bp)		 ++
(10-8 error/bp)※
Sequence
applicability		 Not applicable to GC
rich and repeat sequences		 Not applicable to sequences
that cause cell toxicity		 Applicable to any sequence
Product Linear DNA Circular DNA Circular DNA

※Technology under development to match E. coli cloning.

COMPARISON TABLE (pdf)

06 PRODUCTS & SERVICES

  • 01

    Reagent kits

    Reagent kits for assembly and amplification of large DNA for academia research

    LEARN MORE
  • 02

    Co-development

    Co-development arrangements with partners from the pharma, diagnostics, agriculture and industrial biotech companies

    LEARN MORE
  • 03

    Technology licensing

    Technology licensing to CDMOs and gene synthesis companies for using our proprietary technologies

    LEARN MORE
07 CONTACT
product@oriciro.com
ABOUT US
TEAM & COMPANY
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© 2019 OriCiro Genomics, Inc.

TECHNOLOGY & SERVICES TEAM & COMPANY NEWS
REAGENT KITS
Powerful tool
for genomics research

OriCiro provides reagent kits for assembly and amplification of large DNA up to 200 kbp for research in academia and public institutions.

CO-DEVELOPMENT
Your partner for next-generation genome technologies

OriCiro provides on-demand services to our partners based on our proprietary technologies including a service of creating custom-made libraries of large DNA molecules.

TECHNOLOGY LICENSING
Novel method of manufacturing with an increased efficiency

OriCiro offers a license for use of our cell-free assembly and amplification technologies for the purpose of the development and manufacturing of plasmid DNA and synthetic genes.

REAGENT KITS
Powerful tool for genomics research

OriCiro provides reagent kits for assembly and amplification of large DNA up to 200 kbp for research in academia and public institutions.

CO-DEVELOPMENT
Your partner for next-generation genome technologies

OriCiro provides on-demand services to our partners based on our proprietary technologies including a service of creating custom-made libraries of large DNA molecules.

TECHNOLOGY LICENSING
Novel method of manufacturing with an increased efficiency

OriCiro offers a license for use of our cell-free assembly and amplification technologies for the purpose of the development and manufacturing of plasmid DNA and synthetic genes.

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